Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 36, Issue -, Pages 180-186Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2013.12.004
Keywords
Multiwalled carbon nanotubes; Vertically aligned; Suerhydrophilic; Tritrichomonas foetus; Photodynamic therapy; Tetrasulfonated aluminum phthalocyanine
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Funding
- FAPESP [2008/06654-4, 2011/17877-7, 2011/20345-7]
- CNPq [309699/2009-6]
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Superhydrophilic vertically aligned carbon nanotubes (VACNT-O-2) were used for the first time as scaffolds for photodynamic therapy (PDT) to induce inhibition of cell division in eukaryotic cells. VACNT-O-2 scaffolds were produced on Ti substrates using plasma enhanced chemical vapor deposition technique and functionalized by oxygen plasma. Scanning electron microscopy (SEM) analysis was performed to characterize the surface changes of the protozoan and interaction with VACNT-O-2. Characterization of lipid and total protein expression was performed with protozoa that were or not treated with PDT. Quantification of protein was conducted using Qubit fluorometer and separated on a polyacrylamide gel. SEM analysis showed the release of lipid vesicles by protozoa after the PDT. These vesicles were characterized by the PKH26 fluorescent probe. The results demonstrated a greater amount of protein released after PDT than in the control. When analyzing the protein material in polyacrylamide gel, a significant protein expression of approximately 65 kDa was found. A model identified the programmed death of Tritrichomonas foetus after the PDT was also proposed. (C) 2013 Elsevier B.V. All rights reserved.
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