Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 33, Issue 1, Pages 196-201Publisher
ELSEVIER
DOI: 10.1016/j.msec.2012.08.030
Keywords
Cornea; Collagen; Gelatin; Hyaluronic acid; Tissue engineering; Biocompatible materials
Categories
Funding
- National Basic Research Program of China [2012CB619100]
- National Science Foundation of China [50803018]
- National Science & Technology Pillar Program in the Twelfth Five-Year Plan of China [2012BAI17B02]
- Guangdong Important Scientific and Technological Special Project [2010A080407008]
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Cornea disease may lead to blindness and keratoplasty is considered as an effective treatment method. However, there is a severe shortage of donor corneas worldwide. This paper presents the crosslinked collagen (Col)-gelatin (Gel)-hyaluronic acid (HA) films developed by making use of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as the crosslinker. The test results on the physical and biological properties indicate that the CGH631 film (the mass ratio of Col:Gel:HA = 6:3:1) has appropriate optical performance, hydrophilicity and mechanical properties. The diffusion properties of the CGH631 film to NaCl and tryptophan are also satisfactory and the measured data are 2.43 x 10(-6) cm(2)/s and 7.97 x 10(-7) cm(2)/s, respectively. In addition, cell viability studies demonstrate that the CGH631 film has good biocompatibility, on which human corneal epithelial cells attached and proliferated well. This biocompatible film may have potential use in cornea tissue engineering. (C) 2012 Elsevier B.V. All rights reserved.
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