Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 33, Issue 2, Pages 699-706Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2012.10.021
Keywords
Electrospinning; Mineralization; Poly(D, L-lactide-co-glycolide); Gelatin; Calcium phosphate
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Funding
- Innovation Foundation of Harbin City [2011RFQXS099]
- Postdoctoral Science-Research Developmental Foundation of Heilongjiang Province [LBH-Q09131]
- Fundamental Research Funds for the Central Universities [HEUCFZ1123, HEUCF201210008]
- Natural Science Foundation of Heilongjiang Province [ZD201012]
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Surface mineralization is an effective method to produce calcium phosphate apatite coating on the surface of bone tissue scaffold which could create an osteophilic environment similar to the natural extracellular matrix for bone cells. In this study, we prepared mineralized poly(D,L-lactide-co-glycolide) (PLGA) and PLGA/gelatin electrospun nanofibers via depositing calcium phosphate apatite coating on the surface of these nanofibers to fabricate bone tissue engineering scaffolds by concentrated simulated body fluid method, supersaturated calcification solution method and alternate soaking method. The apatite products were characterized by the scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), and X-ray diffractometry (XRD) methods. A large amount of calcium phosphate apatite composed of dicalcium phosphate dihydrate (DCPD), hydroxyapatite (HA) and octacalcium phosphate (OCP) was deposited on the surface of resulting nanofibers in short times via three mineralizing methods. A larger amount of calcium phosphate was deposited on the surface of PLGA/gelatin nanofibers rather than PLGA nanofibers because gelatin acted as nucleation center for the formation of calcium phosphate. The cell culture experiments revealed that the difference of morphology and components of calcium phosphate apatite did not show much influence on the cell adhesion, proliferation and activity. (C) 2012 Elsevier B.V. All rights reserved.
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