Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 33, Issue 4, Pages 1945-1950Publisher
ELSEVIER
DOI: 10.1016/j.msec.2013.01.001
Keywords
Bombyx mori; Silk fibroin; Electrospinning; Fibroin solubilization; Mechanical properties
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Funding
- INIA (Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Government of Spain)
- FEDER Operative Program of the Region of Murcia
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Silk fibroin (SF) was regenerated using three of the most common protocols described in the bibliography for the dissolution of raw SF (LiBr 9.3 M, CaCl2 50 wt.% or CaCl2:EtOH:H2O 1:2:8 in molar ratio). The integrity of regenerated SF in aqueous solution was analyzed by SDS-PAGE and different profiles of degradation were observed depending on the protocol used. This fact was found to affect also the aqueous solubility of the freeze dried protein. These different SFs were used to produce electrospun mats using SF solutions of SF 17 wt.% in 1,1,1,1',1',1'-hexafluoro-2-propanol (HFIP) and significant differences in fiber sizes, elongation and ultimate strength values were found. This work provides a global overview of the manner that different methods of SF extraction can affect the properties of electrospun SF-mats and consequently it should be considered depending on the use they are going to be made for. (C) 2013 Elsevier B.V. All rights reserved.
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