Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 33, Issue 3, Pages 1242-1246Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2012.12.018
Keywords
Hydroxyapatite; Silver; Thin films; PLO; Antibacterial; Cytotoxicity
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Funding
- Czech Technical University, Prague [SGS12/167/OHK4/2T/17]
- Institutional Research Plan [COST LD 12069, AVOZ50200919]
- Ministry of Education of the Czech Republic [MSM 6198959216]
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Hydroxyapatite layers with silver doping from 0.06 at.% to 14 at.% were prepared by laser deposition. The films' physical properties such as morphology, composition, crystallinity, Young's modulus and microhardness were measured. Films were amorphous or polycrystalline in dependence on deposition temperature (from RT to 600 C). Antibacterial properties were tested using Escherichia coli and Bacillus subtilis cells. The antibacterial efficacy changed with silver doping from 4% to 100%. Cytotoxicity was studied by a direct contact test. Depending on doping and crystallinity the films were either non-toxic or mildly toxic. (C) 2012 Elsevier B.V. All rights reserved.
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