Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 32, Issue 6, Pages 1640-1647Publisher
ELSEVIER
DOI: 10.1016/j.msec.2012.04.055
Keywords
Fe3O4@silica@Au nanoparticles; Glucose biosensor; Electron transfer
Categories
Funding
- Natural Science Foundation of China [20805011, 20905021, 21175218]
- Foundation of the Ministry of Education of China for Returned Scholars
- State Key Laboratory of Analytical Chemistry for Life Science of Nanjing University [SKLACLS1107]
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Monodisperse Fe3O4 magnetic nanoparticles (NPs) were prepared under facile solvothermal conditions and successively functionalized with silica and Au to form core/shell Fe3O4@silica@Au NPs. Furthermore, the samples were used as matrix to construct a glucose sensor based on glucose oxidase (GOD). The immobilized GOD retained its bioactivity with high protein load of 3.92 x 10(-9) mol.cm(-2), and exhibited a surface-controlled quasi-reversible redox reaction, with a fast heterogeneous electron transfer rate of 7.98 +/- 0.6 s(-1). The glucose biosensor showed a broad linear range up to 3.97 mM with high sensitivity of 62.45 mu A.mM(-1) cm(-2) and fast response (less than 5 s). (c) 2012 Elsevier B.V. All rights reserved.
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