Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 31, Issue 2, Pages 262-271Publisher
ELSEVIER
DOI: 10.1016/j.msec.2010.09.008
Keywords
Collagen; Alginate; Self-assembly; Cell culture; Porous architecture
Categories
Funding
- National Natural Science Foundation of China [30670561, 50830105]
- National Basic Research Program of China [2005CB623903, 2007CB936102]
- Sichuan Provincial STP [05 SG022-012]
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Pore architecture and its stable functionality under cell culturing of three dimensional (3D) scaffolds are of great importance for tissue engineering purposes. In this study, alginate was incorporated with collagen to fabricate collagen-alginate composite scaffolds with different collagen/alginate ratios by lyophilizing the respective composite gels formed via collagen fibrillogenesis in vitro and then chemically crosslinking. The effects of alginate amount and crosslinking treatment on pore architecture, swelling behavior, enzymatic degradation and tensile property of composite scaffolds were systematically investigated. The relevant results indicated that the present strategy was simple but efficient to fabricate highly interconnected strong biomimetic 3D scaffolds with nanofibrous surface. NIH3T3 cells were used as a model cell to evaluate the cytocompatibility, attachment to the nanofibrous surface and porous architectural stability in terms of cell proliferation and infiltration within the crosslinked scaffolds. Compared with the mechanically weakest crosslinked collagen sponges, the cell-cultured composite scaffolds presented a good porous architecture, thus permitting cell proliferation on the top surface as well as infiltration into the inner part of 3D composite scaffolds. These composite scaffolds with pore size ranging from 150 to 300 mu m, over 90% porosity, tuned biodegradability and water-uptake capability are promising for tissue engineering applications. (C) 2010 Elsevier B.V. All rights reserved.
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