Journal
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume 29, Issue 5, Pages 1574-1583Publisher
ELSEVIER
DOI: 10.1016/j.msec.2008.12.012
Keywords
Cytocompatibility; Cell culture; Biomaterial; Hydrogel; Poly(vinyl alcohol); Chemical crosslinking
Categories
Funding
- FAPEMIG-PPM/CAPES
Ask authors/readers for more resources
In the present study we report the preparation, characterization and cytocompatibility of novel polymeric systems based on blends of chitosan and poly(vinyl alcohol) (PVA) and chemically crosslinked by glutaraldehyde for biomedical applications. The structure of the hydrogels was characterized through Fourier Transform Infrared spectroscopy (FTIR) and their swelling behavior was investigated as preliminary in vitro test. Bioactivity, cytotoxicity and cell viability were assessed via MTT assay with 2 cell cultures and cell spreading-adhesion analysis. Moreover, the cell viability and potential biocompatibility were assessed by the secretion of nitric oxide by activated macrophages with gamma interferon (IFN-gamma) cytokine and lipopolysaccharide (LPS). It was found that by increasing the chitosan to PVA ratio the swelling behavior was significantly altered. In addition, all tested hydrogels have clearly presented adequate cell viability, non-toxicity and suitable properties which can be tailored for prospective use in tissue engineering. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available