Synthesis, characterization and antibacterial activity studies of poly-{styrene-acrylic acid} with silver nanoparticles

This work reports the preparation and characterization of copolymer poly-(styrene-acrylic acid) with monomeric ratio of styrene/acrylic acid of 9:1 using benzoyl peroxide as initiator and furthermore filled with nanosilver (25 ppm and 50 ppm) in water/acetone (1:40 v/v). The nanosilver emulsion was obtained from chemical reduction using NaBH4 as reducing agent and sodium citrate as the stabilizer. The preparation of nanosilver emulsion was monitored by the appearance of a Plasmon Resonant Absorption band in a UV-visible spectrophotometer and the particles sizes were observed through TEM. Microbiological studies were performed to investigate the antimicrobial activity of this new material against the microorganisms Escherichia coli (ATCC-25922) and Staphylococcus aureus (ATCC-6538), used as reference strains. The antimicrobial activity of the poly-(styrene-acrylic acid) filled with nanosilver was confirmed by the presence of an inhibition halo of the bacterial growth in seeded Culture media, but was not found with the poly (styrene-acrylic acid) alone. The present work suggests that silver ions are released from the polymeric matrix to the culture media and have the ability to tulle the Ag+ ions released by controlling the amount of Ag nanoparticles embedded in the composite. (C) 2008 Elsevier B.V. All rights reserved.