Journal
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume 29, Issue 3, Pages 761-765Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2008.07.010
Keywords
Biosensor; Conducting polymer; Organic electrodes; Bacteriophage; Salmonella
Categories
Funding
- Ontario Ministry of Food and Agriculture Food Safety Research and Innovation Program [TF046807]
- Canadian Commonwealth Fellowship
- Dairy Farmers of Ontario
Ask authors/readers for more resources
There is interest in using bacteriophage as an indicator for the presence of pathogens, such as Salmonella, in health care and food processing environments. However, the current plaque assay technique to detect bacteriophages is time consuming and laboratory based. The following reports on a bacteriophage sensor based on conducting polymer organic electrodes modified with phage host cells (Salmonella Newport). Conducting polymer electrodes were fabricated by chemical deposition of polypyrrole onto the surface of a microporous polycarbonate membrane. The formed films exhibited quasi-reversible redox behaviour which was dominated by anion exchange although cations also contributed to the charge transfer kinetics. Salmonella host cells were absorbed onto the surface of the film and reacted with infecting bacteriophage in LBM broth at 37 degrees C. Upon bacteriophage mediated host cell lysis the impedance of the supporting polypyrrole electrode increased especially in relation to Z ''. It is proposed that the increase in the dielectric properties of the polypyrrole layer was caused by the interaction of cellular constituents derived from the lysed cells. From dose response curves it was found that the sensor could detect 3 log Plaque Forming Units (pfu)/ml within 270 min although no linear correlation between phage concentration and sensor response was observed. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available