Journal
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume 29, Issue 1, Pages 346-349Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2008.07.018
Keywords
Layer-by-layer; Multi-wall carbon nanotubes; Chitosan; Amperometric biosensor
Categories
Funding
- National Science Foundation of China [30772746]
- The Science and Technology Development Plan of Tianjin [06YFGPNC03900]
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A novel amperometric glucose biosensor based on multilayer films containing chitosan, multi-wall carbon nanotubes (MWCNTs) and glucose oxidase (GOD) was developed. MWCNTs were solubilized in chitosan (Chit-MWCNTs) used to interact with GOD. Poly (allylamine) (PAA) and polyvinylsulfuric acid potassium salt (PVS) were alternately deposited on the cleaned Pt electrode surface ((PVS/PAA)(3)/Pt). The (PVS/PAA)(3)/Pt electrode was alternately immersed in Chit-MWCNTs and GOD to assemble different layers of multilayer films. PBS washing was applied at the end of each assembly deposition for dissociating the weak adsorption. Micrographs of MWCNTs were obtained by scanning electron microscope. and properties of the resulting biosensors were measured by electrochemical measurements. Among the resulting biosensors, the biosensor based on eight layers of multilayer films was best. The resulting biosensor was able to efficiently monitor glucose. with the response time within 8 s, a detection limit of 21 mu M estimated at a signal-to-noise ratio of 3. a linear range of 1-10 mM, the sensitivity of 0.45 mu A/mM, and well stability. The study can provide a feasible simple approach on developing a new immobilization matrix for biosensors; and surface functionalization. (C) 2008 Elsevier B.V. All rights reserved.
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