Journal
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume 29, Issue 1, Pages 328-334Publisher
ELSEVIER
DOI: 10.1016/j.msec.2008.07.006
Keywords
Titania nanoparticles; Protamine; Biomimetic synthesis; Yeast alcohol dehydrogenase encapsulation; Stability
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Funding
- National Natural Science Foundation of China [20576096]
- Ministry of Education of China
- Innovative Research Team in University (PCSIRT)
- Program of Introducing Talents of Discipline [B06006]
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This study demonstrates a novel biomimetic approach for the entrapment of yeast alcohol dehydrogenase (YADH) within titania nanoparticles to improve its stability. Protamine was as the template and catalyst for the condensation of titanium (IV) bis(ammonium lactato) dihydroxide (Ti-BALDH) into titania nanoparticles in which YADH was trapped. The as-prepared titania/protamine/YADH composites were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray powder diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The mechanism of YADH encapsulation was tentatively proposed from a series of experimental results. The preliminary investigation showed that encapsulated YADH could retain most of its initial activity. Compared to free YADH. encapsulated YADH exhibited significantly improved thermal, pH and recycling stability. After 5 weeks storage, no substantial loss of catalytic activity for encapsulated YADH was observed. (C) 2008 Elsevier B.V. All rights reserved.
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