Journal
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume 28, Issue 3, Pages 353-358Publisher
ELSEVIER
DOI: 10.1016/j.msec.2007.04.021
Keywords
polycaprolactone; scaffold; tissue engineering; microfabrication; soft lithography; multilayer micromolding
Categories
Ask authors/readers for more resources
Precise surface geometrical morphologies have been shown to improve cellular proliferation, adhesion, and functionality. It has been found that cells respond strongly to feature dimensions a fraction of their size. In this paper, soft lithography techniques were applied to microfabricate polydimethylsiloxane molds with precisely controlled micro-scale patterns. Three-dimensional polycaprolactone (PCL) scaffolds were fabricated using a multilayer micromolding (MMM) method. Proper heating and stamping parameters were developed for micromolding PCL. This process allowed control of the size, shape, and spacing of support structures within the scaffold. The micromolding of multiple layers with independent features allowed for alignment between layers. The high porosity, abundant interconnections, and sharp features were inherent advantages of the scaffolds. Human osteosarcoma cells were seeded in the 3-D scaffolds for cell growth testing. Fluorescent microscopy and scanning electron micrographs showed that cells responded well to the 3-13 scaffolds and the scaffolds regulated cell morphology and adhesion. (c) 2007 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available