Journal
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume 28, Issue 8, Pages 1236-1241Publisher
ELSEVIER
DOI: 10.1016/j.msec.2007.11.004
Keywords
ENFET; Glucose; Dendrimer; Pt nanoparticles; Biosensor
Categories
Funding
- National Natural Science Foundation of China [20676038]
- Ministry of Education [107045]
- key laboratories in Shanghai [05DZ22302, 06DZ22008]
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The biosensors based on ENFETs for direct glucose concentration analysis have been fabricated by introducing dendrimer encapsulated Pt nanoparticles and glucose oxidase (GOx) via it layer-by-layer self-assembly method. The free amine groups located on each poly(amidoamine) dendrimer molecule were exploited to immobilize enzyme through covalent attachment. Depending on metal nanoparticles within dendrimers and biocompatibility of dendrimers, the fabricated glucose sensitive ENFET shows obviously enhanced sensitivity and extended lifetime compared with the conventional ones. The fabricated sensor has a linear range of 0.25-2.0 mM, and a detection limit of ca. 0.15 mM. The influence of buffer concentration, ionic strength and pH was discussed. The biosensor also has good stability, which response could be used for detecting of glucose samples at intervals for at least I month when it stored in dry state at 4 degrees C. (C) 2007 Elsevier B.V. All rights reserved.
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