Journal
MATERIALS LETTERS
Volume 66, Issue 1, Pages 4-6Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.matlet.2011.08.017
Keywords
Self-assembled monolayer; Bioelectrode; Nanoporous Au; Thermal stability
Funding
- JSPS KAKENHI [22656155]
- Grants-in-Aid for Scientific Research [22656155] Funding Source: KAKEN
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Nanoporous Au was fabricated by the dealloying of Au-Ag alloy in nitric acid, and was modified with a self-assembled monolayer (SAM) of 4-aminothiophenol, for the enhancement of high-temperature activity of immobilized laccase. Immobilized laccase exhibited much higher activity than that of free laccase at >45 degrees C. SAM surface modification greatly improved the thermal stability and reusability of immobilized laccase. For example, little degradation in laccase activity when immobilized on SAM-modified nanoporous Au was observed after 2 h incubation at 50 degrees C. This suggested the nanoporous structure and SAM synergistically prevented the conformational change of laccase, and resulted in the enhancement of high-temperature activity. (C) 2011 Elsevier B.V. All rights reserved.
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