Journal
MATERIALS LETTERS
Volume 63, Issue 21, Pages 1876-1879Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.matlet.2009.05.070
Keywords
Gold nanoparticles; E. coli; Pathogen; Antibody
Funding
- Syracuse Center of Excellence CARTI
- U.S. Environmental Protection Agency [X-83232501-0]
Ask authors/readers for more resources
We here describe a method for the simple synthesis of gold nanoparticles (similar to 10 nm in diameter) conjugated with antibody to E. coli O157:H7. Gold nanoparticles with pendant carboxylic acid and alcohol functional groups were synthesized and characterized using transmission electron microscopy (TEM) and infrared spectroscopy. These nanoparticles were then reacted with anti-E. coli O157:H7, using EDC coupling chemistry, and the product was characterized with X-ray photoelectron spectroscopy. Furthermore, binding of the antibody-gold conjugates to E. coli O157:H7 was demonstrated using transmission electron microscopy. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
