Effect of nano- and micro-hydroxyapatite/chitosan-gelatin network film on human gastric cancer cells

After a tumorotomy operation, desirable biomaterials should be able to inhibit the cancer cell growth around the defects. In this article, a comparative study of human gastric cancer SGC-7901 cells behavior on the microhydorxyapatite (mHA) and nano-hydroxyapatite (nHA) surface layer was carried out. nHA crystalline was biomineralized on the chitosan-gelatin network films and the mean size of the nHA crystalline was 17-25 nm evaluated by X-ray diffraction (XRD) and transmission electron microscope (TEM), while the sintered mHA with average size 5 mu m was deposited on the films directly. The surface characteristic observed by scanning electron microscope (SEM). MTT assay of SGC-7901 cells cultured on nHA and mHA surface suggested that nHA crystalline can effectively inhibit the proliferation performance of SGC-7901 cells. The size of HA crystalline has important influence on the adhesion performance and morphology of SGC-7901 cells. (c) 2008 Published by Elsevier B.V.