Journal
RESEARCH IN VETERINARY SCIENCE
Volume 100, Issue -, Pages 8-11Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2015.03.026
Keywords
Intestinal microbiota; Microbial diversity; Rainbow trout; Aquaculture
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Funding
- European Union through the European Regional Development Fund (FEDER)
- Generalitat de Catalunya (Consolidated Research Group: Catalan Institute for Water Research) [SGR 291]
- Ramon y Cajal research fellowship from the Spanish Ministry of Economy and Competitiveness [RYC-2011-08154]
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In this study, we determined the diversity and composition of bacterial communities within the intestinal ecosystem of farmed rainbow trout (Oncorhynchus mykiss). Healthy rainbow trout, weighing between 520 and 750 g, were fed a commercial diet. Subsequently, genomic DNA was isolated from the intestinal mucus (n = 16 fish samples) and combined into groups of four fish samples each for pyrosequencing analysis of bacterial 16S rRNA genes. The results revealed that the most abundant operational taxonomic units (OTUs) were affiliated to the genera Acinetobacter, Cetobacterium, Pseudomonas, and Psychrobacter, and to a lesser extent, the genera Aeromonas, Clostridium, Deefgea, Flavobacterium, Neptuniibacter, and Mycoplasma. These findings could be used as a baseline for further studies about the role of bacterial communities in normal and altered host physiological states. (C) 2015 Elsevier Ltd. All rights reserved.
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