Journal
RESEARCH IN MICROBIOLOGY
Volume 166, Issue 2, Pages 111-120Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2015.01.003
Keywords
Acidophile; Actinobacteria; Iron oxidation; Iron reduction; Novel species
Categories
Funding
- European Union
- Nelsons UK [R35762]
Ask authors/readers for more resources
A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining an abandoned copper mine in north Wales. The isolate (PY-F3) was demonstrated to be a heterotroph that catalyzed the oxidation of ferrous iron (but not of sulfur or hydrogen) under aerobic conditions, and the reduction of ferric iron under micro-aerobic and anaerobic conditions. PY-F3 formed long entangled filaments of cells (>50 mu m long) during active growth phases, though these degenerated into smaller fragments and single cells in late stationary phase. Although isolate PY-F3 was not observed to grow below pH 2.0 and 10 degrees C, harvested biomass was found to oxidize ferrous iron at relatively fast rates at pH 1.5 and 5 degrees C. Phylogenetic analysis, based on comparisons of 16S rRNA gene sequences, showed that isolate PY-F3 has 91-93% gene similarity to those of the four classified genera and species of acidophilic Actinobacteria, and therefore is a representative of a novel genus. The binomial Acidithrix ferrooxidans is proposed for this new species, with PY-F3 as the designated type strain (=DSM 28176(T), =JCM 19728(T)). (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available