Journal
RESEARCH IN MICROBIOLOGY
Volume 166, Issue 1, Pages 1-8Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2014.11.003
Keywords
Lipidomics; Phospholipids; Phosphatidylcholine; Mass spectrometry imaging; HPTLC MALDI TOF MSI; Pseudomonas fluorescens
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Funding
- GRR SeSa (Sanitary Safety Research Network)
- Regional Council of Haute-Normandie (France)
- Conseil general de l'Eure
- Grand Evreux Agglomeration
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Lipids are major functional components of bacterial cells that play fundamental roles in bacterial metabolism and the barrier function between cells and the environment. In an effort to investigate the bacterial lipidome, we adopted a protocol using MALDI-TOF MS imaging coupled to HPTLC to screen a large number of phospholipid classes in a short span of time. With this method, phospholipids of airborne Pseudomonas fluorescens MFAF76a were visualized and identified in sample extracts (measurement accuracy below 0.1 Da, phospholipid identification by means of four characteristic fragment peaks). Via this technique, the P. fluorescens lipidome was shown to comprise three major lipid classes: phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The protocol described herein is simple, rapid and effective for screening of bacterial phospholipid classes. The remarkable presence of a eukaryotic phospholipid, phosphatidylcholine, was observed in P. fluorescens MFAF76a. This lipid is known to play a role in bacteria host interactions and had not been known to be found in P. fluorescens cells. (C) 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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