Journal
REPRODUCTIVE TOXICOLOGY
Volume 56, Issue -, Pages 64-76Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2015.06.050
Keywords
Zebrafish; Zebrafish embryotoxicity test (ZET); Teratogenicity; Morphology scoring system; Harmonization; 3R's
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In the last couple of years, the interest in the zebrafish embryotoxicity test (ZET) for use in developmental toxicity assessment has been growing exponentially. This is also evident from the recent proposal for updating the ICHS5 guideline. The methodology of the ZET used by the different groups varies greatly. To further evaluate its successfulness and to take the ZET to the next level, harmonization of procedures is crucial. In the present study, based on literature and empirical data, the most optimal study design regarding temperature, test chamber, exposure period, presence of chorion, solvent use, exposure method, choice of concentrations, and teratogenic classification is proposed. Furthermore, our morphology scoring system is reported in detail as protocol to further enhance study design harmonization. (C) 2015 Elsevier Inc. All rights reserved.
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