Journal
MASS SPECTROMETRY REVIEWS
Volume 28, Issue 3, Pages 425-447Publisher
WILEY
DOI: 10.1002/mas.20214
Keywords
photodissociation; peptide fragmentation; ultraviolet lasers; MS-MS; tandem MS
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Funding
- National Science Foundation [CHE 0518234, CHE 0431991]
- NIH/NCRR National Center for Glycomics and Glycoproteomics (NCGG) [RR018942]
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR018942] Funding Source: NIH RePORTER
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Mass spectrometric identification of all types of molecules relies on the observation and interpretation of ion fragmentation patterns. Peptides, proteins, carbohydrates, and nucleic acids that are often found as components of complex biological samples represent particularly important challenges. The most common strategies for fragmenting biomolecular ions include low- and high-energy collisional activation, post-source decay, and electron capture or transfer dissociation. Each of these methods has its own idiosyncrasies and advantages but encounters problems with some types of samples. Novel fragmentation methods that can offer improvements are always desirable. One approach that has been under study for years but is not yet incorporated into a commercial instrument is ultraviolet photofragmentation. This review discusses experimental results on various biological molecules that have been generated by several research groups using different light wavelengths and mass analyzers. Work involving short-wavelength vacuum ultraviolet light is particularly emphasized. The characteristics of photofragmentation are examined and its advantages summarized. (C) 2009 Wiley Periodicals, Inc., Mass Spec Rev 28:425-447, 2009
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