Journal
MASS SPECTROMETRY REVIEWS
Volume 28, Issue 2, Pages 254-272Publisher
WILEY
DOI: 10.1002/mas.20200
Keywords
glycosaminoglycan; heparin; heparan sulfate; keratan sulfate; chondroitin sulfate; dermatan sulfate; hyaluronan; mass spectrometry; liquid chromatography; capillary electrophoresis
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Funding
- NIH [P41RR10888, R01HL74197]
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR010888] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL074197, R01HL098950] Funding Source: NIH RePORTER
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The glycosaminoglycan (GAG) family of polysaccharides includes the unsulfated hyaluronan and the sulfated heparin, heparan sulfate, keratan sulfate, and chondroitin/dermatan sulfate. GAGs are biosynthesized by a series of enzymes, the activities of which are controlled by complex factors. Animal cells alter their responses to different growth conditions by changing the structures of GAGs expressed on their cell surfaces and in extracellular matrices. Because this variation is a means whereby the functions of the limited number of protein gene products in animal genomes is elaborated, the phenotypic and functional assessment of GAG structures expressed spatially and temporally is an important goal in glycomics. On-line mass spectrometric separations are essential for successful determination of expression patterns for the GAG compound classes due to their inherent complexity and heterogeneity. Options include size exclusion, anion exchange, reversed phase, reversed phase ion pairing, hydrophilic interaction, and graphitized carbon chromatographic modes and capillary electrophoresis. This review summarizes the application of these approaches to on-line MS analysis of the GAG classes. (C) 2008 Wiley Periodicals, Inc., Mass Spec Rev 28:254-272, 2009
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