Journal
MASS SPECTROMETRY REVIEWS
Volume 28, Issue 5, Pages 703-724Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/mas.20205
Keywords
capillary electrophoresis; mass spectrometry; biomarker; urine; clinical proteomics
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Funding
- British Heart Foundation [BHF/RG/07/005/23633] Funding Source: Medline
- NIDDK NIH HHS [DK071802, R01 DK071802, R01 DK078244, DK064400, DK080301, P01 DK061525, DK078244, R21 DK080301, R24 DK064400, DK061525, R56 DK078244] Funding Source: Medline
- NIGMS NIH HHS [R01 GM080148, R01 GM080148-01A1, R01 GM080148-02, 1R01GM080148] Funding Source: Medline
- Wellcome Trust [066780/2/012] Funding Source: Medline
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Proteome analysis has emerged as a powerful technology to decipher biological processes. One of the main goals is to discover biomarkers for diseases from tissues and body fluids. However, the complexity and wide dynamic range of protein expression present an enormous challenge to separation technologies and mass spectrometry (MS). In this review, we examine the limitations of proteomics, and aim towards the definition of the current key prerequisites. We focus on capillary electrophoresis coupled to mass spectrometry (CEMS), because this technique continues to show great promise. Me discuss CE-MS from an application point of view, and evaluate its merits and vices for biomarker discovery and clinical applications. Finally. we present several examples on the use of CE-MS to determine urinary biomarkers and implications for disease diagnosis, prognosis, and therapy evaluation. (C) 2008 Wiley Periodicals, Inc., Mass Spec Rev 28:703-724, 2009
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