Journal
MARINE POLLUTION BULLETIN
Volume 64, Issue 9, Pages 1782-1788Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2012.05.035
Keywords
CYP4; Perinereis aibuhitensis; Petroleum hydrocarbon; Deltamethrin; Gene expression
Funding
- National Natural Science Foundation of China [30901107, 41106115]
- High technology Research and development program of China [2006AA10Z410]
- National Marine Public Welfare Research Project [200805069]
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A CYP4 cDNA was cloned and characterized to identify the relationship between persistent organic pollutants and stress response in marine polychaete Perinereis aibuhitensis. The full length of PaCYP4 cDNA is 1857 bp and encodes 481 amino acids. The deduced amino acid sequence showed 73% identity with CYP4BB1 from polychaete Nereis virens and shared high homology to other known CYP4 sequences. The expression level of PaCYP4 under petroleum hydrocarbon (PH) and deltamethrin (DM) exposure was detected using Real-time PCR. PH and combined toxicity treatments elevated the mRNA level of PaCYP4 in a dose- and time-dependent manner. The mRNA transcripts of PaCYP4 increased at the beginning of DM exposure and then eventually decreased, and the expression level of PaCYP4 down-regulated with increasing concentration of DM. CYP4 in P. aibuhitensis plays an important role in the metabolism of petroleum hydrocarbon and organochlorine pesticide. (C) 2012 Published by Elsevier Ltd.
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