Journal
MARINE GENOMICS
Volume 7, Issue -, Pages 43-49Publisher
ELSEVIER
DOI: 10.1016/j.margen.2012.03.001
Keywords
Alexandrium tamarense; Alexandrium catenella; Loop-mediated isothermal amplification; Molecular detection; Plankton monitoring; Paralytic shellfish poisoning
Categories
Funding
- Fisheries Research Agency of Japan
Ask authors/readers for more resources
In this study, we succeeded in developing a loop-mediated isothermal amplification (LAMP) method that enables sensitive and specific detection of the toxic marine dinoflagellates Alexandrium tamarense and Alexandrium catenella from single cells of both laboratory cultures and naturally blooming cells within 25 min, by monitoring the turbidimeter from the start of the LAMP reaction. The fluorescence intensity was strong enough to allow discrimination between positive and negative results by naked eye under a UV lamp, even in amplified samples from a single cell, by using the LAMP method. Unambiguous detection by naked eye was possible even in half the volume of LAMP cocktail recommended by the manufacturer, suggesting the potential to significantly reduce the cost of Alexandrium monitoring. Therefore, we can conclude that this method is one of the most convenient, sensitive, and cost-effective molecular tools for Alexandrium monitoring. (C) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available