Journal
MARINE DRUGS
Volume 7, Issue 3, Pages 341-354Publisher
MDPI
DOI: 10.3390/md7030341
Keywords
marine sulfate-reducing bacterium; isolation; identification; broad-spectrum antimicrobial activity; mono-n-butyl phthalate
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Funding
- Chinese National Programs for High Technology Research and Development [2006AA09Z426]
- Scientific and Technological Fund of Dalian [2008J23JH041]
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A marine sulfate-reducing bacterium SRB-22 was isolated by means of the agar shake dilution method and identified as Desulfovibrio desulfuricans by morphological, physiological and biochemical characteristics and 16S rDNA analysis. In the bioassay, its extract showed broad-spectrum antimicrobial activity using the paper disc agar diffusion method. This isolate showed a different antimicrobial profile than either ampicillin or nystatin and was found to produce at least eight antimicrobial components by bioautography. Suitable fermentation conditions for production of the active constituents were determined to be 28 day cultivation at 25 degrees C to 30 degrees C with a 10% inoculation ratio. Under these conditions, the SRB-22 was fermented, extracted and chemically investigated. So far an antimicrobial compound, mono-n-butyl phthalate, and an inactive compound, thymine, have been isolated and characterized.
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