Journal
MARINE BIOTECHNOLOGY
Volume 16, Issue 2, Pages 181-192Publisher
SPRINGER
DOI: 10.1007/s10126-013-9532-1
Keywords
Lactic acid bacteria; Immunomodulation; Shrimp probiotic; Innate immune genes; Disease resistance
Funding
- JSPS Asian CORE Program and Scientific Research [23580257]
- Research and Development Program for New Bio-industry Initiatives, Japan
- Ministry of Education, Culture, Sports, Science and Technology (Monbukagakusho), Japan
- Grants-in-Aid for Scientific Research [23580257] Funding Source: KAKEN
Ask authors/readers for more resources
Fifty-one lactic acid bacteria (LAB) strains were isolated and identified based on 16S ribosomal DNA sequence from the intestinal tracts of 142 kuruma shrimps (Marsupenaeus japonicus) collected from Kanmon Strait, Fukuoka and Tachibana Bay, Nagasaki, Japan. Cellular immunomodulatory function of 51 isolated LAB strains was assessed by measuring the level of interferon (IFN)-gamma induction in mouse spleen cell culture. The strain Lactococcus lactis D1813 exhibited the highest amount of IFN-gamma production and also bactericidal activity and was selected for testing its immunomodulatory role as a probiotic in kuruma shrimp. We also assessed the effect of dietary incorporation of this probiotic on resistance to Vibrio penaeicida infection in the kuruma shrimp. Our results demonstrate that probiotic L. lactis D1813-containing diet-fed (10(5) cfu g(-1)) shrimps displayed a significant up-regulation of lysozyme gene expressions in the intestine and hepatopancreas. However, insignificantly higher expression of anti-lipopolysaccharide factor, super oxide dismutase, prophenoloxidase, and toll-like receptor 1 was recorded in the intestine of shrimps fed the probiotic diet. Moreover, significantly increased (P < 0.01) resistance to the bacterial pathogen in term of better post-infection survival (61.7 %) was observed in the shrimps fed with the probiotic-incorporated diet compared with the control diet-fed group (28.3 %). The present study indicates the immunomodulatory role of the LAB L. lactis D1813 on the kuruma shrimp immune system and supports its potential use as an effective probiotic in shrimp aquaculture.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available