Journal
MARINE BIOTECHNOLOGY
Volume 14, Issue 3, Pages 253-260Publisher
SPRINGER
DOI: 10.1007/s10126-011-9414-3
Keywords
Metagenomic cloning; alpha-Amylase; Glycoside hydrolase family GH13; Subfamily classification
Funding
- Natural Science Foundation of Anhui Province, China [11040606M65]
- Ministry of Education, China [211073]
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A gene encoding a starch-hydrolyzing enzyme was isolated from a marine metagenomic library and overexpressed in Escherichia coli. The enzyme, designated AmyP, shows very low similarity to full-length sequences of known alpha-amylases, although a catalytic domain correlated with the alpha-amylase superfamily was identified. Based on the range of substrate hydrolysis and the product profile, the protein was clearly defined as a saccharifying-type alpha-amylase. Sequence comparison indicated that AmyP was related to four putative glycosidases previously identified only in bacterial genome sequences. They were all from marine bacteria and formed a new subfamily of glycoside hydrolase GH13. Moreover, this subfamily was closely related to the probable genuine bacterial alpha-amylases (GH13_19). The results suggested that the subfamily may be an independent clade of ancestral marine bacterial alpha-amylases.
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