Journal
MAIN GROUP CHEMISTRY
Volume 7, Issue 2, Pages 97-107Publisher
IOS PRESS
DOI: 10.1080/10241220802200347
Keywords
Ni(II) complexes; phenanthroline; bipyridine; DNA binding; cleavage
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Mixed ligand complexes of the type [Ni(phen)(2)(bnp)](PF6)(2) center dot 2H(2)O (1) and [Ni(bpy)(2)(bnp)](PF6)(2) center dot 2H(2)O (2) (where phen, 1,10,Phenanathroline; bpy, 2,2 bipyridine; bnp, benzo[1.8]naphthyridinone-2-ol) have been synthesized and characterized by employing analytical and spectral methods. The DNA binding properties of the complexes with calf thymus-DNA were studied by using absorption spectra, viscosity measurements as well as thermal denaturation experiments. The absorption spectra indicated that the Ni(II) complexes intercalate tightly between the base pairs of the DNA with intrinsic DNA binding constants of 1.9 x 10(4) M (1) for (1) and 2.5 x 10(4) M-1 for (2) in 5 mM Tris-HCl/50 mM NaCl buffer at pH 7.2, respectively. The large enhancement in the relative viscosity of DNA on binding to the bnp supports the proposed DNA binding modes. The oxidative cleavage and photo-cleavage activity of complexes (1) and (2) were studied. The photo-induced DNA cleavage activities of the complexes in the presence and absence of different additives were studied by using UV light at 365 nm. The results show significant nuclease activity even in the absence of additives at 60 mu M concentration.
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