4.5 Article

Monitoring Bone Marrow-Originated Mesenchymal Stem Cell Traffic to Myocardial Infarction Sites Using Magnetic Resonance Imaging

Journal

MAGNETIC RESONANCE IN MEDICINE
Volume 65, Issue 5, Pages 1430-1436

Publisher

WILEY-BLACKWELL
DOI: 10.1002/mrm.22735

Keywords

murine model; myocardial infarction; mesenchymal stem cells; magnetic resonance imaging; fluorescent imaging

Funding

  1. American Health Assistance Foundation (National Heart Foundation)

Ask authors/readers for more resources

How stem cells promote myocardial repair in myocardial infarction (MI) is not well understood. The purpose of this study was to noninvasively monitor and quantify mesenchymal stem cells (MSC) from bone marrow to MI sites using magnetic resonance imaging (MRI). MSC were dual-labeled with an enhanced green fluorescent protein and micrometer-sized iron oxide particles prior to intra-bone marrow transplantation into the tibial medullary space of C57BI/6 mice. Micrometer-sized iron oxide particles labeling caused signal attenuation in T-2*-weighted MRI and thus allowed noninvasive cell tracking. Longitudinal MRI demonstrated MSC infiltration into MI sites over time. Fluorescence from both micrometer-sized iron oxide particles and enhanced green fluorescent protein in histology validated the presence of dual-labeled cells at MI sites. This study demonstrated that MSC traffic to MI sites can be noninvasively monitored in MRI by labeling cells with micrometer-sized iron oxide particles. The dual-labeled MSC at MI sites maintained their capability of proliferation and differentiation. The dual-labeling, intra-bone marrow transplantation, and MRI cell tracking provided a unique approach for investigating stem cells' roles in the post-MI healing process. This technique can potentially be applied to monitor possible effects on stem cell mobilization caused by given treatment strategies. Magn Reson Med 65:1430-1436, 2011. (C) 2011 Wiley-Liss, Inc.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available