Journal
MAGNETIC RESONANCE IN MEDICINE
Volume 63, Issue 5, Pages 1383-1390Publisher
WILEY
DOI: 10.1002/mrm.22313
Keywords
nanoparticles; dendritic cells; T2-weighted imaging; lymph nodes; immunotherapy
Funding
- NIA [AG026366-01A1]
- Department of Radiology
- NATIONAL CANCER INSTITUTE [P50CA128323] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [T32EB001628] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [K01AG026366] Funding Source: NIH RePORTER
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We report the development of superparamagnetic iron oxide (SPIOs) nanoparticles and investigate the migration of SPIO-labeled dendritic cells (DCs) in a syngeneic mouse model using magnetic resonance (MR) imaging. The size of the dextran-coated SPIO is roughly 30 nm, and the DCs are capable of independent uptake of these particles, although not at levels comparable to particle uptake in the presence of a transfecting reagent. On average, with the assistance of polylysine, the particles were efficiently delivered inside DCs within one hour of incubation. The SPIO particles occupy approximately 0.35% of cell surface and are equivalent to 34.6 pg of iron per cell. In vivo imaging demonstrated that the labeled cells migrated from the injection site in the footpad to the corresponding popliteal lymph node. The homing of labeled cells in the lymph nodes resulted in a signal drop of up to 79%. Furthermore, labeling DCs with SPIO particles did not compromise cell function, we demonstrated that SPIO-enhanced MR imaging can be used to track the migration of DCs effectively in vivo. Magn Reson Med 63:1383-1390, 2010. (C) 2010 Wiley-Liss, Inc.
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