Journal
MAGNETIC RESONANCE IN MEDICINE
Volume 62, Issue 3, Pages 747-753Publisher
WILEY
DOI: 10.1002/mrm.22063
Keywords
in vivo cytometry; F-19 MRI; adaptive immunity; antigen-specific T cells; perfluoropolyether
Funding
- National Institutes of Health [R01-EB003453, R01-EB004155, P01-HD047675]
- National Institute of Biomedical Imaging and Bioengineering [P41EB-001977]
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [P01HD047675] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [P41EB001977, R01EB003453, R01EB004155] Funding Source: NIH RePORTER
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Noninvasive methods to image the trafficking of phenotypically defined immune cells are paramount as we attempt to understand adaptive immunity. A F-19 MRI-based methodology for tracking and quantifying cells of a defined phenotype is presented. These methods were applied to a murine inflammation model using antigen-specific T cells. The T cells that were intracellularly labeled ex vivo with a perfluoropolyether (PFPE) nanoemulsion and cells were transferred to a host receiving a localized inoculation of antigen. Longitudinal F-19 MRI over 21 days revealed a dynamic accumulation and clearance of T cells in the lymph node (LN) draining the antigen. The apparent T-cell numbers were calculated in the LN from the time-lapse F-19 MRI data. The effect of in vivo T-cell division on the F-19 MRI cell quantification accuracy was investigated using fluorescence assays. Overall, in vivo cytometry using PFPE labeling and F-19 MRI is broadly applicable to studies of whole-body cell biodistribution. Magn Reson Med 62:747-753, 2009. (C) 2009 Wiley-Liss, Inc.
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