Journal
MAGNETIC RESONANCE IN MEDICINE
Volume 59, Issue 6, Pages 1225-1231Publisher
WILEY
DOI: 10.1002/mrm.21606
Keywords
SPIO; molecular imaging; contrast agent; gene expression; reporter gene
Funding
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [F31EB005928] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM008169] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS064991] Funding Source: NIH RePORTER
- NHLBI NIH HHS [U01HL89711] Funding Source: Medline
- NIBIB NIH HHS [F31EB005928, F31 EB005928] Funding Source: Medline
- NIGMS NIH HHS [T32 GM008169] Funding Source: Medline
- NINDS NIH HHS [R01 NS064991] Funding Source: Medline
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Magnetic resonance imaging (MRI) is routinely used to obtain anatomical images that have greatly advanced biomedical research and clinical health care today, but the full potential MRI in providing functional, physiological, and molecular information is only beginning to emerge. In this work, we sought provide a gene expression marker for MRI based on bacterial magnetosomes, tiny magnets produced by naturally occurring magnetotactic bacteria. Specifically, magA, a gene in magnetotactic bacteria known to be involved with iron transport, expressed in a commonly used human cell line, 293FT, resulting in the production of magnetic, iron-oxide nanoparticles by these cells and leading to increased transverse relaxivity. MRI shows that these particles can be formed in vivo utilizing e dogenous iron and can be used to visualize cells positive for magA. These results demonstrate that magA alone is sufficient to produce magnetic nanoparticles and that it is an appropriate candidate for an MRI reporter gene.
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