Journal
MAGNETIC RESONANCE IN MEDICINE
Volume 60, Issue 1, Pages 1-7Publisher
WILEY-BLACKWELL
DOI: 10.1002/mrm.21622
Keywords
manganese oxide; iron oxide; cellular imaging; contrast agent; transplantation; nanoparticles
Funding
- National Research Foundation of Korea [R16-2002-003-01001-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- NIBIB NIH HHS [R21 EB005252, K01 EB 006394, K01 EB006394-02, K01 EB006394, R01 EB015031, R21 EB005252-03, R21 EB 005252] Funding Source: Medline
- NINDS NIH HHS [R01 NS 045062, R01 NS045062] Funding Source: Medline
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Rat glioma cells were labeled using electroporation with either manganese oxide (MnO) or superparamagnetic iron oxide (SPIO) nanoparticles. The viability and proliferation of SPIO-labeled cells (1.9 mg Fe/ml) or cells electroporated with a low dose of MnO (100 mu g Mn/ml) was not significantly different from unlabeled cells; a higher MnO dose (785 mu g Mn/ml) was found to be toxic. The cellular ion content was 0.1-0.3 pg Mn/cell and 4.4 pg Fe/cell, respectively, with cellular relaxivities of 2.5-4.8 s(-1) (RI) and 45-84 s(-1) (R-2) for MnO-labeled cells. Labeled cells (SPIO and low-dose MnO) were each transplanted in contralateral brain hemispheres of rats and imaged in vivo at 9.4T. While SPIO-labeled cells produced a strong negative contrast due to the increase in R-2, MnO-Iabeled cells produced positive contrast with an increased R-1. Simultaneous imaging of both transplants with opposite contrast offers a method for MR double labeling of different cell populations.
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