Technical and practical aspects of 19F NMR-based screening: toward sensitive high-throughput screening with rapid deconvolution

The technical and practical aspects of 19F NMR-based screening against a macromolecular target are analyzed in detail. A novel method utilizing the relaxation of 19F homonuclear double quantum coherence is proposed for performing NMR-based binding assays in a direct- or competition-mode format. A combined strategy based on 19F NMR chemical shift prediction, 2D 19F NMR DOSY, and 2D 19F1H NMR long-range COSY experiments is presented for the deconvolution of complex mixtures of fluorinated molecules generated by either addition of single compounds or by chemical synthesis. The approaches presented here allow the screening of complex mixtures, even in the case where the exact composition is not known, and the rapid identification of the binders contained in the mixtures. Copyright (c) 2012 John Wiley & Sons, Ltd.