Journal
MACROMOLECULES
Volume 42, Issue 7, Pages 2360-2367Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ma8022712
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Funding
- National Science Foundation [CAREER CHE-0645793]
- Christopher S. Foote Graduate Fellowship
- Alfred P. Sloan Foundation
- Amgen
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We describe a straightforward approach to synthesize polymers with end-groups that bind site-specifically to two different proteins. Telechelic biotin-maleimide poly(N-isopropylacrylamide) (pNIPAAm) was synthesized for the formation of streptavidin (SAv)-bovine serum albumin (BSA) polymer conjugates. Reversible addition-fragmentation chain transfer (RAFT) polymerization of NIPAAm was conducted in the presence of biotinylated chain transfer agents (CTAs) with either ester or amide linkages, and the resultant a-biotinylated pNIPAAms were formed with low polydispersity indices (PDI <= 1.09). UV-vis analysis of the trithiocarbonate chain-ends indicated 88% or greater retention of the group. A malemide was introduced to the omega chain-end via a radical cross-coupling reaction with a functionalized azo-initiator. The polymer structures were characterized by H-1 NMR spectroscopy and gel permeation chromatography (GPC). The resultant biotin-maleimide heterotelechelic polymer was used to form a SAv-BSA heterodimer conjugate. Bioconjugate formation was confirmed by gel electrophoresis.
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