Journal
MACROMOLECULAR RAPID COMMUNICATIONS
Volume 35, Issue 9, Pages 868-874Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/marc.201400027
Keywords
biocatalysis; chemical transformation; peptide hydrogel; self-assembly
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Funding
- BBSRC
- EPSRC
- Biotechnology and Biological Sciences Research Council [BB/E010717/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J020192/1, EP/K016210/1] Funding Source: researchfish
- BBSRC [BB/E010717/1] Funding Source: UKRI
- EPSRC [EP/J020192/1, EP/K016210/1] Funding Source: UKRI
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Enzymes are attractive, green alternatives to chemical catalysts within the industrial sector, but their robustness to environmental conditions needs optimizing. Here, an enzyme is tagged chemically and recombinantly with a self-assembling peptide that allows the conjugate to spontaneously assemble with pure peptide to form -sheet-rich nanofibers decorated with tethered enzyme. Above a critical concentration, these fibers entangle and form a 3D hydrogel. The immobilized enzyme catalyzes chemical transformations and critically its stability is increased significantly where it retains activity after exposure to high temperatures (90 degrees C) and long storage times (up to 12 months).
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