Journal
MACROMOLECULAR RAPID COMMUNICATIONS
Volume 34, Issue 9, Pages 785-790Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/marc.201200809
Keywords
semiconducting polymer dots; ultrasensitive detection; western blot; dot blot
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Funding
- National Institutes of Health [GM085485]
- National Science Foundation [CHE-0924320]
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Ultrasensitive fluorescence imaging of proteins on western blots using a bright, compact, and orange-emitting semiconducting polymer dot (CN-PPV) is demonstrated. A detection limit at the single-picogram level in dot blots is achieved; with conventional western blotting, 50 pg of transferrin and trypsin inhibitor after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transfer onto a polyvinylidene fluoride membrane are detected. This method does not require any additional equipment or time compared with the conventional procedure with traditional fluorescent probes.
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