Journal
MACROMOLECULAR RAPID COMMUNICATIONS
Volume 29, Issue 15, Pages 1293-1297Publisher
WILEY-BLACKWELL
DOI: 10.1002/marc.200800248
Keywords
enzymatic polymerization; glycogen branching enzyme; (1)H NMR; phosphorylase; polysaccharides; synthesis
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An enzymatic tandem reaction is described in which the enzymes phosphorylase and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) catalyze the synthesis of branched polyglucans from glucose-1-phosphate (G-1-P). Phosphorylase consumes G-1-P and polymerizes linear amylose while DR GBE introduces branching points on the alpha-(1 -> 6) positions by reshuffling short oligosaccharides. The resulting branched polyglucans have an unusually high degree of branching of 11%.
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