Journal
MACROMOLECULAR CHEMISTRY AND PHYSICS
Volume 212, Issue 1, Pages 64-71Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/macp.201000448
Keywords
biodegradable; cross-linking; hyperbranched; intracellular gene delivery; stimuli-sensitive polymers
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Funding
- National Natural Science Foundation of China [20774068]
- Ph.D. Programs Foundation of Ministry of Education of China [20070486035]
- National Key Basic Research Program of China [2005CB623903, 2009CB930300]
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Reducible disulfide-containing hyperbranched PEI-SS-HP was synthesized via click chemistry and evaluated as nonviral gene carrier. The structure of the polymers was confirmed by H-1 NMR and FTIR. It was shown that the PEI-SS-HP was able to bind plasmid DNA to positively charged nanoparticles. The reduction sensitivity of the PEI-SS-HP was confirmed by gel retardation assay in the presence of DTT mimicking an intracellular reductive environment. In vitro experiments revealed that the reducible PEI-SS-HP not only had much lower cytotoxicity, but also posed superior transfection activity as compared to non-degradable 25-kDa PEI. The results indicate that a reducibly degradable PEI-SS-HP can be a promising gene vector.
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