Journal
MACROMOLECULAR CHEMISTRY AND PHYSICS
Volume 211, Issue 4, Pages 404-411Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/macp.200900514
Keywords
drug delivery system; nanoparticles; poly(ethylene glycol); poly(lactide-co-glycolide); self-assembly
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Funding
- Spanish Ministry of Science and Innovation [MAT 2007-60458]
- Natural Science Foundation of China [50873087]
- Major State Basic Research Program of China [2005CB623902]
- National High-tech Research and Development Program [2006AA03Z442, 2006AA02A140]
- Spanish Ministry of Science arid Innovation
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The layer-by-layer (LbL) assembly technique was applied for the surface modification of biodegradable poly(lactide-co-glycolide) nanoparticles (NPs), employing poly(acrylic acid) (PAA), and polyethylenimine (PEI) as building blocks. Amino terminated poly(ethylene glycol) (PEG) and folate decorated PEG (PEG-FA) were grafted onto the multilayers via condensation between carboxylic groups and amine groups from PEG or PEG-FA. The LbL assembly and the covalent functionalization were monitored by means of zeta-potential measurements and the quartz crystal microbalance with dissipation technique (QCM-D). Protein adsorption after incubation of the NPs in culture medium containing optionally the serum proteins was investigated and related to cellular uptake. Experiments on cellular uptake showed that after PEGylation the uptake ratio of the NPs decreased significantly, but became three times larger when PEG-FA was grafted on the NPs instead of the PEG.
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