Journal
MACROMOLECULAR BIOSCIENCE
Volume 14, Issue 11, Pages 1619-1626Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201400295
Keywords
encapsulation; hydrophobic amino acids; peptide synthesis; protease; silica
Funding
- RIKEN Biomass Engineering Program
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We report the encapsulation of three different proteases in bioinspired silica. Silica particles were formed under mild reaction conditions using cationic amine-rich ethyleneamines as initiators, which resulted in aggregations of nanoscale spheres. Following encapsulation, the proteases were characterized for their hydrolytic and aminolytic activities. The encapsulation resulted in an increase in the thermal stability of the proteases for both hydrolysis and aminolysis reactions. The enhanced thermal stability of the encapsulated proteases increased the production of poly-L-leucine by aminolysis. Furthermore, the encapsulation of papain resulted in an increase in the production of poly-L-alanine and poly-L-valine at 50 degrees C.
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