Journal
MACROMOLECULAR BIOSCIENCE
Volume 14, Issue 7, Pages 1015-1024Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201400004
Keywords
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Funding
- Chinese Program for New Century Excellent Talents in Universities [NCET-11-0661]
- National Natural Science Foundation of China [21174054, 21004030]
- Scientific Research Foundation for Returned Scholars from Ministry of Education of China
- Natural Science Foundation of Anhui Province of China [B1408085MB26]
- Academic and Technology Introduction Project of Anhui University of China [AU02303203]
- US National Institutes of Health [R01CA129189]
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A new type of homopolymers, PMAOE, bearing acid-cleavable cationic side-chains is synthesized and characterized. PMAOE is obtained via free radical polymerization, and they could efficiently bind and condense plasmid DNA at neutral pH. The strength of DNA binding is dependent on the length of PMAOE chains. NMR analysis reveals that hydrolysis of the ortho ester group of PMAOE follows an exocyclic mechanism and the rate of hydrolysis is much accelerated at mildly acidic pH, leading to accelerated disruption of polyplexes and release of DNA in mildly acidic environment. PMAOE is not toxic to cultured NIH 3T3 and COS-7 cells measured by MTT. This study demonstrates a unique mechanism of achieving pH-modulated binding and release of DNA from polymers with potential applications for gene delivery.
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