Journal
MACROMOLECULAR BIOSCIENCE
Volume 14, Issue 3, Pages 334-339Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201300425
Keywords
biomembrane adhesive; cell immobilization; choline phosphate; LCST
Funding
- CIHR
- CBS
- CFI
- Michael Smith Foundation for Health Research (MSFHR)
- MSFHR
Ask authors/readers for more resources
A new monomer, 2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethyl methacrylate (AEO(4)MA), and its direct atom transfer radical polymerization (ATRP) into poly(AEO(4)MA), then clicked with prop-2-ynyle choline phosphate (CP) to produce a poly(choline phosphate) are described. This polymer exhibits a lower critical solution temperature (LCST) at approximate to 32 degrees C, and provides a universal thermally reversible biomembrane adhesive, which can rapidly both bind to any mammalian cell membrane and internalize into the cytoplasm of nucleated cells below the LCST. Moving above the LCST reverses cell surface binding. The use of ATRP implies that such polymers can be applied to modify the surfaces of a wide range of biomaterials. The capacity to bind and immobilize cells at room temperature and release them above the LCST should be particularly useful for in vitro cell manipulation and tissue engineering applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available