Journal
MACROMOLECULAR BIOSCIENCE
Volume 13, Issue 9, Pages 1221-1227Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201300139
Keywords
photoenhanced gene transfection; poly(L-lysine) dendrimers; porphyrin; star polymers
Funding
- National Natural Science Foundation of China [21104099, 31271019]
- Fundamental Research Funds for the Central Universities [21613410]
- Natural Science Foundation of Guangdong Province [S2011010004545]
- Science and Technology Projects of Guangdong Province [2011B031300006]
- Key Project of Scientific and Technical Innovation for Universities in Guangdong Province [cxzd1109]
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A star-shaped polymer (PP-PLLD) consisting of a porphyrin (PP) core and poly(L-lysine) dendron arms (PLLD) is synthesized by the click reaction, and its ability to deliver pEGFP is investigated in this paper. It is found that PP-PLLD has a good buffer capacity and can form compact complexes with pEGFP. In vitro assay indicates that PP-PLLD shows photoenhanced gene transfection efficiency. PP-PLLD consisting of only third generation PLLD shows a higher transfected cell number than PEI under a Xe lamp at the N/P ratio of 20, and meanwhile shows a neglectable cytotoxicity to HeLa cells. Therefore, PP-PLLD with suited irradiation is a promising nontoxic and photoinducible effective gene delivery strategy, which should be encouraged in gene therapy.
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