Journal
MACROMOLECULAR BIOSCIENCE
Volume 13, Issue 11, Pages 1478-1484Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201300129
Keywords
biotin; cellular delivery; graphene oxide; surface functionalization; Tris-NTA
Funding
- CSIR
- UGC, India
- DST, India
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A versatile method of dual chemical functionalization of graphene oxide (GO) with Tris-[nitrilotris(acetic acid)] (Tris-NTA) and biotin for cellular delivery of oligohistidine- and biotin-tagged biomolecules is reported. Orthogonally functionalized GO surfaces with Tris-NTA and biotin to obtain a dual-functionalized GO (DFGO) are prepared and characterized by various spectroscopic and microscopic techniques. Fluorescence microscopic images reveal that DFGO surfaces are capable of binding oligohistidine-tagged biomolecules/proteins and avidin/biotin-tagged biomolecules/proteins orthogonally. The DFGO nanoparticles are non-cytotoxic in nature and can deliver oligohistidine- and biotin-tagged biomolecules simultaneously into the cell.
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