Journal
MACROMOLECULAR BIOSCIENCE
Volume 12, Issue 7, Pages 946-951Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201100351
Keywords
alginate hydrogels; cells; double emulsions; encapsulation; microfluidics
Funding
- International Network of Emerging Science and Technology
- Purdue Research Fund
- Hanyang University [HY-2011-N]
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A microfluidic technique is described to encapsulate living cells in alginate hydrogel microparticles generated from monodisperse double-emulsion templates. A microcapillary device is used to fabricate double emulsion templates composed of an alginate drop surrounded by a mineral oil shell. Hydrogel formation begins when the alginate drop separates from the mineral oil shell and comes into contact with Ca2+ ions in the continuous phase. Alginate hydrogel microparticles with diameters ranging from 60 to 230?mu m are obtained. 65% of the cells encapsulated in the alginate microparticles were viable after one week. The technique provides a useful means to encapsulate the living cells in monodisperse hydrogel microparticles.
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