Journal
MACROMOLECULAR BIOSCIENCE
Volume 12, Issue 6, Pages 794-804Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201100519
Keywords
biodegradable; hyperbranched; non-viral gene delivery; polylysine; transient gene expression
Funding
- European commission [NMP4-CT-2006-02556]
Ask authors/readers for more resources
The feasibility of a new transfection agent, HBPL, for the production of recombinant IgG antibody via TGE as well as for the transfection of primary cells is studied. Under the conditions investigated, transfection of CHO-DG44 cells using HBPL results in IgG yields that are comparable to those obtained with PEI. In experiments with CHO-K1 cells and MEFs the use of HPBL allows to achieve transfection efficiencies comparable to or better than those obtained with PEI or Fugene (R). HBPL-mediated transfection does not require complex pre-formation, works well in serum-containing media and is biodegradable, which may prevent cumulative cytotoxicity and facilitates downstream processing.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available