Journal
MACROMOLECULAR BIOSCIENCE
Volume 13, Issue 2, Pages 203-214Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201200344
Keywords
antibody attachment; HPMA copolymers; RAFT-polymerizations; targeting moieties
Funding
- university center SAMT
- university center COMATT
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Herein the synthesis of antibodypolymer conjugates, with a quite narrow dispersity based on the polymer HPMA, are reported. These conjugates are synthesized by coupling antibodies to maleimide-functionalized poly(N-(2-hydroxypropyl)-methacrylamide) (poly-HPMA) copolymers derived through reversible addition-fragmentation chain transfer (RAFT) polymerization of pentafluorophenyl methacrylate via the intermediate step of an activated ester polymer. We develop a protocol that allows the attachment of two different model antibodies, monoclonal anti-RAGE (receptor for advanced glycation end-products) antibody, and polyclonal human immunoglobulin (huIgG). Modification of the antibody and conjugation is monitored by SDS-PAGE electrophoresis. Preserved affinity is demonstrated by Western Blott and cell-uptake analysis, for example, to cells of the immune system.
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